《植物生理学报》 2016, 52(5): 645-652

云南樱桃优良单株的组织培养与快速繁殖

叶小玲¹, 胡晓敏², 叶超宏³, 唐伟洲⁴, 沈荔荔⁵, 邓小梅^4,*

¹广州天适集团有限公司, 广州510335; ²广东天适樱花悠乐园有限公司, 广州510920; ³广州旺地园林工程有限公司, 广州510335; ⁴华南农业大学林学与风景园林学院, 广东省森林植物种质创新与应用重点实验室, 广州510642; ⁵英德市旺地樱花种植有限公司, 广东英德513000

通信作者：邓小梅；E-mail: dxmei2006@scau.edu.cn

摘　要：

以云南樱桃优株‘广州’樱的半木质化嫩枝为外植体, 采用丛生芽发生途径, 建立其组培快繁体系。结果表明: 最佳腋芽诱导培养基为改良MS (针对云南樱桃组织培养配制的基本培养基)+1.0 mg•L^-1 6-苄氨基嘌呤(6-BA)+0.1 mg•L^-1萘乙酸(NAA)+30 g•L^-1蔗糖+6 g•L^-1琼脂, 诱导率高达100%; 最适增殖培养基为改良MS+1.0 mg•L^-1 6-BA+0.05 mg•L^-1 NAA+0.2 mg•L^-1吲哚丁酸(IBA)+30 g•L^-1蔗糖+6 g•L^-1琼脂, 继代周期15 d, 增殖系数达4.72; 最适生根培养基为1/2改良MS+0.5 mg•L^-1 NAA+0.5 mg•L^-1 IBA+15 g•L^-1蔗糖+6 g•L^-1琼脂, 15 d生根率达100%, 平均每株苗生根5条以上。炼苗后, 移栽于泥炭土:珍珠岩:蛭石=2:1:1 (V/V/V)的混合基质中, 成活率达90%以上。

关键词：云南樱桃; 组织培养; 基本培养基; 快速繁殖

收稿：2016-03-08   修定：2016-04-12

资助：广东省省级科技型中小企业技术创新专项(2014A010101097)和广东省省级科技计划项目(2014A030304004)。

Tissue culture and rapid micropropagation of superior individual of Cerasus yunnanensis

YE Xiao-Ling¹, HU Xiao-Min², YE Chao-Hong³, TANG Wei-Zhou⁴, SHEN Li-Li⁵, DENG Xiao-Mei^4,*

¹Tianshi Group Co., Ltd., Guangzhou 510335, China; ²Tianshi Cherry Amusement Garden Co., Ltd., Guangzhou 510920, China; ³Wangdi Landscape Architecture Co., Ltd., Guangzhou 510335, China; ⁴College of Forestry and Landscape Architecture, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China; ⁵Yingde Wangdi Cerasus Plantation Co., Ltd., Yingde, Guangdong 513000, China

Corresponding author: DENG Xiao-Mei; E-mail: dxmei2006@scau.edu.cn

Abstract:

In this paper, an economic and high effective technology system for rapid propagation of Cerasus yunnanensis was established, through multiple-shoots proliferation. The explants were taken from the late and half lignificational stems with shoot in the adult selected trees. The results show that the optimal inducing medium was modified MS+1.0 mg•L^-1 6-BA +0.1mg•L^-1 NAA+30 g•L^-1 sucrose+6 g•L^-1 gelatin; the optimal multiplication medium was modified MS+1.0 mg•L^-1 6-BA+0.05 mg•L^-1 NAA+0.2 mg•L^-1 IBA+30 g•L^-1 sucrose+6 g•L^-1 gelatin, the subculture cycle was 15 d, and the highest multiplication rate was 4.72; the optimal rooting medium was 1/2 modified MS+0.5 mg•L^-1 NAA+0.5 mg•L^-1 IBA+15g•L^-1 sucrose+6 g•L^-1 gelatin, and the rooting rate has reached 100% within 15 d. The transplanting matrix were peat, pearlite and vermiculite (3:1:1, V/V/V), and its corresponding transplanting survival rate was over 90%.

Key words: Cerasus yunnanensis; tissue culture; basal medium; rapid propagation